NPM1/FLT3 ANALYSIS

Acute myeloid leukaemia (AML) is an extremely heterogeneous disease. The WHO classifies AML into numerous categories depending on the cytogenetic abnormalities. This helps in stratification of patients for treatment. In normal karyotype AMLs, FLT3 internal tandem duplications (ITDs) and NPM1 mutations are frequently observed and identifies patients with poor and good prognosis, respectively. An AML that carries both FLT3-ITD and NPM1 mutations has intermediate risk. FLT3– tyrosine kinase domain (TKD) mutations may also be seen in AML. The impact of these mutations is however, controversial.

FLT3-ITD is caused by in-frame insertions and duplications in the FLT3 gene. NPM1 mutations are caused by 4bp insertions that disrupt the nucleolar localization signal. Both these types of mutations can easily be screened by fragment analysis. The point mutations in codons 835/836 of FLT3-TKD are screened by PCR and restriction fragment length polymorphism (RFLP).

 

NPM1-FLT3 ANALYSIS
Figure 1: FLT3 and NPM1 mutation analysis in AML

 

References

  1. Qin Huang et al, British Journal of Haematology 2008, 142,482-492.
  2. Kottaridis et al., Blood 2002, 100, (7); 2393-2398
  3. Spiekermann et al., Blood 2002, 100; 3423-3425
  4. Schneider F et al Blood 2009: 113(21): 5250-5253

Note : The above methodology describes the approach to diagnosis followed in CMC, Vellore. This is not a recommendation or guidelines, participants are free to choose their own methodology(ies).

Participation Fee : 2000 INR

Participation Fee - As package

If you choose to participate in assessments by choosing them as a package, here is the pricing. You can choose any ONE package and can choose any other assessment individually that does not include in your chosen package.

Package 1

7,000 INR Per EQAS

  • Haemophilia A
  • Haemophilia B
  • Beta Thalassemia
  • Alpha Thalassemia
  • Thrombophilia

Package 2

5,500 INR Per EQAS

  • JAK2 V617F
  • NPM1/FLT3 ANALYSIS
  • RT PCR BCR-ABL1/PML-RARA

Package 3

9,000 INR Per EQAS

  • Haemophilia A
  • Haemophilia B
  • Beta Thalassemia
  • Alpha Thalassemia
  • Thrombophilia
  • JAK2 V617F
  • NPM1/FLT3 ANALYSIS
  • RT PCR BCR-ABL1/PML-RARA

Package 4

12,500 INR Per EQAS

  • All the assessments
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